全文获取类型
收费全文 | 221篇 |
免费 | 6篇 |
专业分类
林业 | 31篇 |
农学 | 7篇 |
54篇 | |
综合类 | 7篇 |
农作物 | 13篇 |
水产渔业 | 19篇 |
畜牧兽医 | 77篇 |
园艺 | 4篇 |
植物保护 | 15篇 |
出版年
2023年 | 1篇 |
2022年 | 1篇 |
2021年 | 3篇 |
2020年 | 1篇 |
2019年 | 3篇 |
2018年 | 4篇 |
2017年 | 2篇 |
2016年 | 8篇 |
2015年 | 7篇 |
2014年 | 8篇 |
2013年 | 18篇 |
2012年 | 14篇 |
2011年 | 21篇 |
2010年 | 9篇 |
2009年 | 11篇 |
2008年 | 20篇 |
2007年 | 18篇 |
2006年 | 16篇 |
2005年 | 12篇 |
2004年 | 13篇 |
2003年 | 13篇 |
2002年 | 11篇 |
2001年 | 8篇 |
2000年 | 2篇 |
1999年 | 2篇 |
1998年 | 1篇 |
排序方式: 共有227条查询结果,搜索用时 156 毫秒
221.
Nagase N Shimizu A Kawano J Yamashita K Yoshimura H Ishimaru M Kojima A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(12):1169-1172
Fifty-three strains of Staphylococcus aureus isolated from cows affected with mastitis from 21 prefectures in Japan were characterized by phenotypic and genotypic methods. Thirty-three (62.3%) strains showed biotype K-beta+CV:A, coagulase type VI, and sensitivity to bovine phages of group III or IV. These 33 strains could be subdivided into two groups on the basis of the production of staphylococcal enterotoxins (SEs) and on toxic shock syndrome toxin-1 (TSST-1). By pulsed-field gel electrophoresis, the 16 SEC- and TSST-1-producing strains showed similar patterns that differed by only a few fragments, suggesting that they were genetically closely related. Fifteen of 17 non SEC-producing strains which did not produce any other SEs and TSST-1 were genetically different from the SEC-producing strains and showed genetic diversity. 相似文献
222.
Fukata T Naito F Yoshida N Yamaguchi T Mizumura Y Hirai K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(11):1079-1080
A total of 1,013 feces samples and 8 mesenteric lymphonodus samples obtained from apparently healthy dogs were examined for the incidence of salmonella infection. One strain of S. typhimurium (ST) was isolated from feces of one dog, and S. enteritidis (SE) was isolated from the mesenteric lymphonodus of one dog. Sera obtained from 330 apparently healthy dogs were examined for Salmonella antibodies using an ELISA with heated whole cells of SE and ST. Fifty-one of the 330 serum samples were considered to be positive for salmonella antibodies, including 12 which were SE-positive and 39 which were ST-positive. These results indicate that dogs cause possible environmental problems as Salmonella carriers. 相似文献
223.
Makoto Kimura Naoko Takahashi-Ando Takumi Nishiuchi Takeshi Tokai Makoto Fujimura Hiroshi Hamamoto Isamu Yamaguchi 《Pesticide biochemistry and physiology》2006,86(3):117-123
Fusarium head blight (FHB) is a devastating disease of important cereal crops resulting in significant yield loss and mycotoxin contamination. Persistent outbreaks of FHB in Europe and North America have led to various efforts to understand the mechanisms of resistance to this disease and mycotoxin biosynthesis. In this minireview, we summarize basic and applied studies conducted in our laboratories into reducing mycotoxin contamination in FHB. 相似文献
224.
225.
Mo CHEN Li-Yi CAI Naoko KANNO Takako KATO Jinxing LU Fan JIN Honghua WANG Masayo SEKITA Masashi HIGUCHI Saishu YOSHIDA Hideji YAKO Hiroki UEHARU Shun-Ichiro IZUMI Yukio KATO 《The Journal of reproduction and development》2013,59(5):457-462
Recently we demonstrated an ectopic expression of the human herpesvirus 1 thymidine
kinase (HHV1-TK) gene by functioning of an intrinsic endogenous promoter in the
transgenic rat (TG-rat), suggesting that HHV1 infection in humans induces expression
of the TK gene with the ectopic promoter in the testis and results in accumulation of
HHV1-TK protein, triggering male infertility similar to that in the TG-rat. Hence, in
this study, we started to investigate a relationship between infection of herpesvirus
and human male infertility. Semen was donated by Chinese male infertile patients (153
men, aged 21–49 years) with informed consent, followed by DNA preparation and
analysis by PCR and DNA sequencing. Semen volume, sperm number and density, and sperm
motility were examined. DNAs of HHV1, HHV4, HHV5 and HHV6 were confirmed by PCR,
electrophoresis and DNA sequencing. Finally, virus DNA was identified in 59 patients
(39%). The number of carriers was 39 (25%) for HHV1, 6 (4%) for HHV4, 33 (22%) for
HHV5 and 3 (2%) for HHV6, respectively. Moreover, double-infection was found in 22
out of 59 specimens (37%), most of which were double-infection of HHV1 and HHV5 (15
out of 22 carriers). Though slight severity was present in some of the carriers, the
relationship between virus infection and sperm impairment was not conclusive.
Accordingly, it is essential to examine whether the viral HHV1-TK gene is expressed
in the testis of the infertile human HHV carrier. 相似文献
226.
Takasao N Tsuji-Naito K Ishikura S Tamura A Akagawa M 《Journal of agricultural and food chemistry》2012,60(5):1193-1200
The breakdown of collagenous networks with aging results in hypoactive changes in the skin. Accordingly, reviving stagnant collagen synthesis can help protect dermal homeostasis against aging. We searched for type I collagen biosynthesis-inducing substances in various foods using human dermal fibroblasts and found that cinnamon extract facilitates collagen biosynthesis. Cinnamon extract potently up-regulated both mRNA and protein expression levels of type I collagen without cytotoxicity. We identified cinnamaldehyde as a major active component promoting the expression of collagen by HPLC and NMR analysis. Since insulin-like growth factor-I (IGF-I) is the most potent stimulator of collagen biosynthesis in fibroblasts, we examined the effect of cinnamaldehyde on IGF-I signaling. Treatment with cinnamaldehyde significantly increased the phosphorylation levels of the IGF-I receptor and its downstream signaling molecules such as insulin receptor substrate-1 and Erk1/2 in an IGF-I-independent manner. These results suggested that cinnamon extract is useful in antiaging treatment of skin. 相似文献
227.
Otolith microchemical analyses of the strontium (Sr) and calcium (Ca) concentrations in the eels Anguilla japonica and A. anguilla caught in Tokyo Bay were undertaken to reconstruct the eels’ migratory histories. A. japonica in the yellow stage (immature stage) were caught in a bay without any adjacent rivers or streams. A. anguilla was in the silver stage (early maturing stage), and the eel was confirmed to have just begun spawning migration to the open
ocean from Tokyo Bay based on the otolith Sr:Ca ratios, which showed a typical catadromous life history with low Sr:Ca ratio
values throughout the eel’s life after recruitment. The mean Sr:Ca ratios in A. japonica from the elver mark to the otolith edge indicated the eels belonged to several general categories of migratory histories,
including sea eels (average Sr:Ca ratio ≥6.0 × 10−3) and estuarine eels (average Sr:Ca ratio 2.5 to 6.0 × 10−3) based on the criteria reported previously in A. japonica. All eels had a certain freshwater life period, although the period was highly variable among fish. These results indicate
that A. japonica has a flexible pattern of migration, with the ability to adapt to various habitats and salinities. 相似文献